An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency-Reference-Cited by-同舟云学术

An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency

Published:2022-12-21 Issue:6 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Yang Yuqing¹²,Liu Menghui¹,Wang Tianqi¹,Wang Qian¹,Liu Huaiwei¹^ORCID,Xun Luying¹³^ORCID,Xia Yongzhen¹^ORCID

Affiliation:

1. State Key Laboratory of Microbial Technology, Shandong University, Qingdao, People’s Republic of China

2. Institute of Marine Science and Technology, Shandong University, Qingdao, People’s Republic of China

3. School of Molecular Biosciences, Washington State University, Pullman, Washington, USA

Abstract

DNA transformation is commonly used in cloning; however, high transformation efficiency becomes a limiting factor in many applications, such as the construction of CRISPR and DNA libraries, the assembly of multiple fragments, and the transformation of large plasmids. We developed a new competent cell preparation method with unmatched transformation efficiency.

Funder

MOST | National Key Research and Development Program of China

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02497-22

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