Qualification of a Biolayer Interferometry Assay to Support AZD7442 Resistance Monitoring-Reference-Cited by-同舟云学术

Qualification of a Biolayer Interferometry Assay to Support AZD7442 Resistance Monitoring

Published:2022-10-26 Issue:5 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Brady Tyler¹,Zhang Tianhui²,Tuffy Kevin M.¹,Haskins Nantaporn³,Du Qun³,Lin Jia³,Kaplan Gilad³,Novick Steven²,Roe Tiffany L.¹,Ren Kuishu⁴,Rosenthal Kim⁴,McTamney Patrick M.⁴,Abram Michael E.¹^ORCID,Streicher Katie¹,Kelly Elizabeth J.¹^ORCID

Affiliation:

1. Translational Medicine, Vaccines and Immune Therapies, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, Maryland, USA

2. Data Sciences and Quantitative Biology, AstraZeneca, Gaithersburg, Maryland, USA

3. Biologics Engineering, R&D, AstraZeneca, Gaithersburg, Maryland, USA

4. Virology and Vaccine Discovery, Vaccines and Immune Therapies, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, Maryland, USA

Abstract

SARS-CoV-2 variants harbor multiple substitutions in their spike trimers, potentially leading to breakthrough infections and clinical resistance to immune therapies. For this reason, a BLI assay was developed and qualified to evaluate the reliability of screening SARS-CoV-2 spike trimer variants against anti-SARS-CoV-2 monoclonal antibodies (MAbs) tixagevimab and cilgavimab, the components of AZD7442, prior to in vitro pseudovirus neutralization susceptibility verification testing.

Funder

AstraZeneca

DOD | Defense Advanced Research Projects Agency

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01034-22

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