Standardization of SARS-CoV-2 Cycle Threshold Values: Multisite Investigation Evaluating Viral Quantitation across Multiple Commercial COVID-19 Detection Platforms-Reference-Cited by-同舟云学术

Standardization of SARS-CoV-2 Cycle Threshold Values: Multisite Investigation Evaluating Viral Quantitation across Multiple Commercial COVID-19 Detection Platforms

Published:2023-02-14 Issue:1 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Gavina Kenneth¹²^ORCID,Franco Lauren C.¹,Robinson Christopher M.³^ORCID,Hymas Weston⁴,Lei Guang-Sheng¹,Sinclair Will⁵,Hall Tara⁵,Carlquist John⁵,Lavik John-Paul¹²,Emery Christopher L.¹²,Heaton Phillip R.⁶,Hillyard David⁴,Lopransi Bert K.⁵,Relich Ryan F.¹²^ORCID

Affiliation:

1. Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA

2. Division of Clinical Microbiology, Indiana University Health, Indianapolis, Indiana, USA

3. Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA

4. ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA

5. Intermountain Laboratory Services, Department of Pathology, Intermountain Healthcare, Salt Lake City, Utah, USA

6. Department of Pathology and Laboratory Medicine, Health Partners, Bloomington, Minnesota, USA

Abstract

From the onset of the COVID-19 pandemic, the demand for SARS-CoV-2 testing has resulted in an explosion of analytical tests with very different approaches and designs. The variability in testing modalities, compounded by the lack of available commercial reference materials for standardization early in the pandemic, has led to several challenges regarding data harmonization for viral quantitation.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.04470-22

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1. Personal characteristics and transmission dynamics associated with SARS-CoV-2 semi-quantitative PCR test results: an observational study from Belgium, 2021–2022;Frontiers in Public Health;2024-09-10

2. Improving the quality of quantitative polymerase chain reaction experiments: 15 years of MIQE;Molecular Aspects of Medicine;2024-04

3. Reporting SARS-CoV-2 viral load from upper respiratory tract specimens in the post-emergency phase: a narrative review;Journal of Public Health and Emergency;2023-12