Affiliation:
1. Department of Environmental Health Science, University of Georgia, Athens, Georgia, USA
Abstract
Prior research has suggested that the use of
Aliivibrio fischeri
-derived donor plasmids with the pES213 origin of replication may provide increased plasmid stability for the tagging of vibrios compared to
Escherichia coli
-derived p15A plasmids. Here, we present a structured protocol for conjugation-based tagging of vibrios using the pES213-derived plasmid pVSV102 and evaluate the plasmid stability of tagged strains.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology
Cited by
6 articles.
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