Recombinant HNP-1 Produced by Escherichia coli Triggers Bacterial Apoptosis and Exhibits Antibacterial Activity against Drug-Resistant Bacteria-Reference-Cited by-同舟云学术

Recombinant HNP-1 Produced by Escherichia coli Triggers Bacterial Apoptosis and Exhibits Antibacterial Activity against Drug-Resistant Bacteria

Published:2022-02-23 Issue:1 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Xie Qi¹²³,Wang Yin¹²^ORCID,Zhang Mengmeng⁴,Wu Shujia¹²,Wei Wei²,Xiao Weidi²,Wang Yihao²,Zhao Jinchao¹,Liu Nan¹,Jin Yiguang⁴,Wu Junzhu¹,Xu Ping¹²⁵⁶

Affiliation:

1. School of Basic Medical Science, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery of Ministry of Education and Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China

2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, China

3. Department of Neurology, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing, China

4. Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing, China

5. Anhui Medical University, Hefei, China

6. School of Public Health, China Medical University, Shenyang, China

Abstract

Human neutrophil peptide-1 (HNP-1) is a promising antibiotic candidate, but its clinical application has been hampered by the difficulty of mass production and an inadequate understanding of its bactericidal mechanisms. In this study, we demonstrated that recombinant protein expression combined with ultrafiltration may be a simple and cost-effective solution to HNP-1 production.

Funder

Chinese National Basic Research Programs

National Megaprojects for Key Infectious Diseases

Foundation of State Key Lab of Proteomics

Guangzhou Science and technology innovation & development project

Innovation Foundation of Medicine

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link