Rapid Assessment of SARS-CoV-2 Variant-Associated Mutations in Wastewater Using Real-Time RT-PCR-Reference-Cited by-同舟云学术

Rapid Assessment of SARS-CoV-2 Variant-Associated Mutations in Wastewater Using Real-Time RT-PCR

Published:2023-02-14 Issue:1 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Farkas Kata¹²^ORCID,Pellett Cameron¹,Williams Rachel¹,Alex-Sanders Natasha¹,Bassano Irene³⁴,Brown Mathew R.³⁵,Denise Hubert³,Grimsley Jasmine M. S.³⁶,Kevill Jessica L.¹,Khalifa Mohammad S.³⁷,Pântea Igor¹,Story Rich³⁸,Wade Matthew J.³⁵,Woodhall Nick¹,Jones Davey L.¹⁹

Affiliation:

1. Centre for Environmental Biotechnology, School of Natural Sciences, Bangor University, Bangor, Gwynedd, United Kingdom

2. School of Ocean Sciences, Bangor University, Menai Bridge, Anglesey, United Kingdom

3. UK Health Security Agency, Environmental Monitoring for Health Protection, London, United Kingdom

4. Department of Infectious Disease, Imperial College London, London, United Kingdom

5. School of Engineering, Newcastle University, Newcastle-upon-Tyne, United Kingdom

6. The London Data Company, London, United Kingdom

7. Division of Biosciences, College of Health, Medicine and Life Sciences, Brunel University, London, United Kingdom

8. Servita Professional Services (UK) Ltd., London, United Kingdom

9. Food Futures Institute, Murdoch University, Murdoch, Western Australia, Australia

Abstract

With the global spread of COVID-19, it is essential to identify emerging variants which may be more harmful or able to escape vaccines rapidly. To date, the gold standard to assess variants circulating in communities has been the sequencing of the S gene or the whole genome of SARS-CoV-2; however, that approach is time-consuming and expensive.

Funder

UK Government Accelerated Capacity Environment

EC | European Regional Development Fund

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.03177-22

Reference67 articles.

1. WHO. 2020. WHO Coronavirus (COVID-19) Dashboard. https://covid19.who.int/. Retrieved 22 June 2022.

2. Genomic characterisation of an emergent SARS-CoV-2 lineage in Manaus: preliminary findings;Faria NR;Virological,2021