Cell-Free Dot Blot: an Ultra-Low-Cost and Practical Immunoassay Platform for Detection of Anti-SARS-CoV-2 Antibodies in Human and Animal Sera-Reference-Cited by-同舟云学术

Cell-Free Dot Blot: an Ultra-Low-Cost and Practical Immunoassay Platform for Detection of Anti-SARS-CoV-2 Antibodies in Human and Animal Sera

Published:2023-04-13 Issue:2 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Norouzi Masoud¹^ORCID,Truong Thang²^ORCID,Jaenes Katariina¹,Warner Bryce M.²,Vendramelli Robert²,Tierney Kevin²,Kobasa Darwyn²,Tailor Nikesh³,Plant Pamela⁴,dos Santos Claudia⁴⁵,Babiuk Shawn³,Ambagala Aruna³^ORCID,Pardee Keith¹⁶^ORCID

Affiliation:

1. Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario, Canada

2. Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

3. National Centre for Foreign Animal Diseases, Canadian Food Inspection Agency, Winnipeg, Manitoba, Canada

4. Keenan Research Centre for Biomedical Science, St. Michael’s Hospital, Unity Health Toronto, Toronto, Ontario, Canada

5. Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada

6. Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada

Abstract

The recent COVID-19 pandemic has highlighted the need for diagnostic platforms that are rapidly adaptable, affordable, and accessible globally, especially for low-resource settings. To address this need, we describe the development and functional validation of a novel immunoassay technique termed the cell-free dot blot (CFDB) method.

Funder

CIHR Foundation Grant Program

CIHR Canada Research Chair Program

Canadian Safety and Security Program

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02457-22

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