Culture-Free Phylogenetic Analysis of Legionella pneumophila Using Targeted CRISPR/Cas9 Next-Generation Sequencing-Reference-Cited by-同舟云学术

Culture-Free Phylogenetic Analysis of Legionella pneumophila Using Targeted CRISPR/Cas9 Next-Generation Sequencing

Published:2022-08-31 Issue:4 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Domazetovska Ana¹²³^ORCID,Jensen Slade O.⁴³^ORCID,Gray Matthew²,Radzieta Michael⁴³,Maley Michael¹²

Affiliation:

1. Department of Microbiology and Infectious Diseases, Liverpool Hospital, Liverpool, New South Wales, Australia

2. NSW Health Pathology, Microbiology, Liverpool Hospital, Liverpool, New South Wales, Australia

3. Antibiotic Resistance and Mobile Elements Group, Ingham Institute of Applied Medical Research, Sydney, Australia

4. Infectious Diseases and Microbiology, School of Medicine, Western Sydney University, Sydney, Australia

Abstract

The bacterium Legionella pneumophila is responsible for outbreaks of serious and life-threatening pneumonia called Legionnaires’ disease. There is a need for new molecular methods that allow investigation of Legionella outbreaks directly from patient samples, without the need for prior microbiological culture, which causes delays.

Funder

Department of Microbiology and Infectious Diseases, Liverpool Hospital, Liverpool, New South Whales, Australia

Sydney Partnership for Health, Education, Research & Enterprise - Maridulu Budyari Gumal

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.00359-22

Reference38 articles.

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2. Real-time investigation of a Legionella pneumophila outbreak using whole genome sequencing

3. Genomic Characterization of a Large Outbreak of Legionella pneumophila Serogroup 1 Strains in Quebec City, 2012

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5. Genome Sequencing Links Persistent Outbreak of Legionellosis in Sydney (New South Wales, Australia) to an Emerging Clone of Legionella pneumophila Sequence Type 211

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