Persistent Nonviral Plasmid Vector in Nasal Tissues Causes False-Positive SARS-CoV-2 Diagnostic Nucleic Acid Tests-Reference-Cited by-同舟云学术

Persistent Nonviral Plasmid Vector in Nasal Tissues Causes False-Positive SARS-CoV-2 Diagnostic Nucleic Acid Tests

Published:2022-12-21 Issue:6 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Beck Ingrid A.¹,Styrchak Sheila¹,Miller Leslie¹,Mast Fred¹^ORCID,Vigdorovich Vladimir¹^ORCID,Yeung Winnie¹,Ko Daisy¹,Oldroyd Alyssa¹,Hardy Samantha¹,Li Song¹,Houck John¹,Jiang Yonghou¹,Dambrauskas Nicholas¹,Darcey Catherine¹,Raappana Andrew¹,Selman William¹,Sather D. Noah¹²³,Aitchison John D.¹²⁴,Harrington Whitney E.¹²³,Frenkel Lisa M.¹²³⁵⁶^ORCID

Affiliation:

1. Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, Washington, USA

2. Department of Pediatrics, University of Washington, Seattle, Washington, USA

3. Department of Global Health, University of Washington, Seattle, Washington, USA

4. Department of Biochemistry, University of Washington, Seattle, Washington, USA

5. Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA

6. Department of Medicine, University of Washington, Seattle, Washington, USA

Abstract

Asymptomatic laboratory workers who tested positive for SARS-CoV-2 for days to months were found to harbor a laboratory plasmid vector containing SARS-CoV-2 DNA, which they had worked with in the past, in their nasal secretions. While prior studies have documented contamination of research personnel with PCR amplicons, our observation is novel, as these individuals shed the laboratory plasmid over days to months, including during isolation in their homes.

Funder

Seattle Children's Research Institute

HHS | NIH | National Institute of Allergy and Infectious Diseases

Burroughs Wellcome Fund

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01695-22

Reference10 articles.

1. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets

2. Amplicon residues in research laboratories masquerade as COVID-19 in surveillance tests

3. Laboratory-Generated DNA Can Cause Anomalous Pathogen Diagnostic Test Results

4. Laboratory Worker Self-Contamination with Noninfectious SARS-CoV-2 DNA Can Result in False-Positive Reverse Transcriptase PCR-Based Surveillance Testing

5. Evaluation of DNA Plasmid Storage Conditions

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