Ability of the MeltPro MTB/PZA Assay to Detect Susceptibility to Pyrazinamide in Rifampin-Resistant Tuberculosis Patients

Author:

Li Rong12,Li Yang2,Chen Xinchang2,Jia Lina3,Yu Hongying4,Huang Ya5,Wu Qianhong6,Xiao Mingying7,Ge Shijia2,Zhang Yilin2,Feng Zhen2,Li Qingge8ORCID,Xu Ye8,Shi Wenzhi8,Sun Feng2ORCID,Zhang Wenhong1291011

Affiliation:

1. Department of Infectious Diseases, Jing’an District Central Hospital, Fudan University, Shanghai, China

2. Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China

3. Department of Tuberculosis, Guiyang Public Health Clinical Center, Guiyang, China

4. Center for Infectious Diseases, The First People’s Hospital of Huaihua, Huaihua, Hunan, China

5. Department of Tuberculosis, Ge Jiu Infectious Disease Hospital, Gejiu, China

6. Department of Tuberculosis, Shaanxi Provincial Tuberculosis Prevention and Control Hospital, Xi’an, Shannxi, China

7. Department of Tuberculosis, Baoshan People’s Hospital, Baoshan, China

8. Engineering Research Centre of Molecular Diagnostics of the Ministry of Education, State Key Laboratory of Cellular Stress Biology, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China

9. National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, China

10. Key Laboratory of Medical Molecular Virology (MOE/MOH), Shanghai Medical College, Fudan University, Shanghai, China

11. Shanghai Huashen Institute of Microbes and Infections, Shanghai, China

Abstract

This study evaluated the accuracy of the MeltPro MTB/PZA assay at detecting the presence of PZA resistance through registered clinical trials. Compared to DNA sequencing, the assay had high sensitivity and negative predictive value, suggesting its potential utility as a screening tool in clinical practice.

Funder

Clinical Research Plan of SHDC

National Natural Science Foundation of China

Shanghai Sailing Program

Shanghai Municipal Science, Technology Major Project

Wenhong Zhang Station in Yunnan Province

Shanghai Science and Technology Committee

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

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