Novel <i>In Situ</i> Hybridization Assay for Chromogenic Single-Molecule Detection of Human Papillomavirus E6/E7 mRNA-Reference-Cited by-同舟云学术

Novel In Situ Hybridization Assay for Chromogenic Single-Molecule Detection of Human Papillomavirus E6/E7 mRNA

Published:2023-04-13 Issue:2 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Rao Xuelian¹,Zheng Liangkai²,Wei Kaipeng³,Li Meiqing²,Jiang Meng¹,Qiu Jianlong³,Zhou Yulin⁴,Ke Rongqin¹^ORCID,Lin Chen¹

Affiliation:

1. School of Medicine, Huaqiao University, Xiamen, Fujian, China

2. Department of Pathology, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, China

3. Department of Pathology, The 910th Hospital, Quanzhou, Fujian, China

4. United Diagnostic and Research Center for Clinical Genetics, Women and Children’s Hospital, School of Medicine and School of Public Health, Xiamen University, Xiamen, Fujian, China

Abstract

In situ detection of viral mRNA expression in tissue samples is of great value for pathological diagnosis to access viral infection status. Unfortunately, conventional RNA in situ hybridization assays lack sensitivity and specificity for clinical diagnostic purposes.

Funder

福建省科学技术厅 | Science Fund for Distinguished Young Scholars of Fujian Province

泉州市科技局 | Quanzhou City Science and Technology Program

Huaqiao University

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.03896-22

Reference23 articles.

1. Immunohistochemistry Innovations for Diagnosis and Tissue-Based Biomarker Detection