Usefulness of Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry in the Characterization of <i>Leishmania</i> Strains Causing Tegumentary Leishmaniasis in Bolivia versus <i>hsp70</i> Gene Sequencing-Reference-Cited by-同舟云学术

Usefulness of Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry in the Characterization of Leishmania Strains Causing Tegumentary Leishmaniasis in Bolivia versus hsp70 Gene Sequencing

Published:2023-02-14 Issue:1 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Torrico Mary Cruz¹²³^ORCID,Fernández-Arévalo Anna³,Ballart Cristina³⁴,Solano Marco¹,Rojas Ernesto¹,Abras Alba⁵^ORCID,Gonzales Fabiola¹,Arnau Albert³⁵,Tebar Silvia³,Llovet Teresa⁶⁷,Lozano Daniel¹²,Ariza-Vioque Eva³⁸,Gascón Joaquim⁴⁹,Picado Albert⁴,Torrico Faustino¹²,Muñoz Carmen⁶⁷¹⁰,Gállego Montserrat³⁴⁹

Affiliation:

1. Facultad de Medicina, Universidad Mayor de San Simón, Cochabamba, Bolivia

2. Fundación CEADES y Medio Ambiente, Cochabamba, Bolivia

3. Secció de Parasitologia, Departament de Biologia, Sanitat i Medi Ambient, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Barcelona, Spain

4. Instituto de Salud Global de Barcelona (ISGlobal), Barcelona, Spain

5. Departament de Biologia, Universitat de Girona, Girona, Spain

6. Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau Barcelona, Barcelona, Spain

7. Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain

8. Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain

9. CIBERINFEC, ISCIII-CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III

10. Institut de Recerca Biomèdica Sant Pau, Barcelona, Spain

Abstract

The objective of the study was to evaluate the usefulness of MALDI-TOF MS for the characterization of Leishmania species circulating in Bolivia, in comparison with the sequencing of the hsp70 gene. In our study, all of the isolates could be identified, and no misidentifications were observed at the complex level.

Funder

MAEC | Agencia Española de Cooperación Internacional para el Desarrollo

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.03477-22

Reference47 articles.

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2. Organización Panamericana de la Salud. 2021. Leishmaniasis. Informe epidemiológico de las Américas. N° 10. https://iris.paho.org/handle/10665.2/55344.

3. Ministerio de Salud de Bolivia. 2015. Norma nacional y manual de procedimientos técnicos de leishmaniasis Documentos Técnicos Normativos N° 364. La Paz. https://www.minsalud.gob.bo/images/Documentacion/dgss/Epidemiologia/Leishmaniasis/365-Norma_Nacional_y_Manual_de_Procedimientos_Tcnicos_de_Leishmaniasis-2015.pdf.

4. Ministerio de Salud de Bolivia. 2017. Programa Nacional de Vigilancia y control de Leishmaniasis, p 51–54. In Anuario Epidemiología 2016. Ministerio de Salud. https://www.minsalud.gob.bo/component/jdownloads/?task=download.send&id=99&catid=9&m=0&Itemid=646.

5. American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples

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