Efficient Autotransporter-Mediated Extracellular Secretion of a Heterologous Recombinant Protein by Escherichia coli-Reference-Cited by-同舟云学术

Efficient Autotransporter-Mediated Extracellular Secretion of a Heterologous Recombinant Protein by Escherichia coli

Published:2023-06-15 Issue:3 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Beriotto Irene¹²,Icke Christopher³,Sevastsyanovich Yanina R.¹,Rossiter Amanda E.¹,Romagnoli Giacomo²,Savino Silvana²,Hodges Freya J.³,Cole Jeffrey A.¹,Saul Allan²,MacLennan Calman A.²⁴,Cunningham Adam F.¹⁵^ORCID,Micoli Francesca²,Henderson Ian R.¹³^ORCID

Affiliation:

1. Institute of Microbiology and Infection, University of Birmingham, Birmingham, United Kingdom

2. GSK Vaccines Institute for Global Health Srl, Siena, Italy

3. Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia

4. Jenner Institute, University of Oxford, Oxford, United Kingdom

5. Institute for Immunology and Immunotherapy, University of Birmingham, Birmingham, United Kingdom

Abstract

Recombinant protein production is an essential component of the biotechnology sector. Here, we show that the autotransporter platform is a viable method for the recombinant production, secretion, and purification of a “difficult” to produce protein on an industrially relevant scale. Use of the autotransporter platform could reduce the number of downstream processing operations required, thus accelerating the development time and reducing costs for recombinant protein production.

Funder

EC | FP7 | People | FP7 People: Marie-Curie Actions

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.03594-22

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