Usefulness of Combining Sputum and Nasopharyngeal Samples for Viral Detection by Reverse Transcriptase PCR in Adults Hospitalized with Acute Respiratory Illness-Reference-Cited by-同舟云学术

Usefulness of Combining Sputum and Nasopharyngeal Samples for Viral Detection by Reverse Transcriptase PCR in Adults Hospitalized with Acute Respiratory Illness

Published:2022-12-21 Issue:6 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Kim Keun Ju¹²^ORCID,Yun Seung Gyu¹²^ORCID,Cho Yunjung¹²^ORCID,Nam Myung-Hyun¹²^ORCID,Lee Chang Kyu¹²^ORCID

Affiliation:

1. Department of Laboratory Medicine, Korea University Anam Hospital, Seoul, South Korea

2. Department of Laboratory Medicine, Korea University College of Medicine, Seoul, South Korea

Abstract

This study reveals that RT-PCR utilizing sputum significantly increased the detection rate for respiratory viral nucleic acids among adult patients admitted to the hospital, compared to nasopharyngeal swabs (NPS). Notably, combined samples of sputum and NPS maintained the majority of the improved sputum detection rate with only a few positive signal losses from NPS samples.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02775-22

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