Whole-Genome Sequencing-Based Re-Identification of <i>Pseudomonas putida</i> / <i>fluorescens</i> Clinical Isolates Identified by Biochemical Bacterial Identification Systems-Reference-Cited by-同舟云学术

Whole-Genome Sequencing-Based Re-Identification of Pseudomonas putida / fluorescens Clinical Isolates Identified by Biochemical Bacterial Identification Systems

Published:2022-04-27 Issue:2 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Tohya Mari¹²,Teramoto Kanae³,Watanabe Shin²,Hishinuma Tomomi¹,Shimojima Masahito⁴⁵,Ogawa Miho⁵,Tada Tatsuya¹,Tabe Yoko⁶,Kirikae Teruo¹^ORCID

Affiliation:

1. Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan

2. Department of Microbiome Research, Juntendo University School of Medicine, Tokyo, Japan

3. Koichi Tanaka Mass Spectrometry Research Laboratory, Kyoto, Japan

4. SUGIYAMA-GEN Co., Ltd., Tokyo, Japan

5. BML, Inc., Saitama, Japan

6. Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan

Abstract

Most of the clinical isolates, identified as P. putida or P. fluorescens , were misidentified in clinical laboratories. Whole-genome sequencing (WGS) revealed that these isolates belonged to different Pseudomonas species, including novel species. WGS is a gold-standard method to identify Pseudomonas species, and MALDI-TOF MS analysis has the potential to complement WGS to reliably identify them.

Funder

MEXT | Japan Society for the Promotion of Science

Japan Agency for Medical Research and Development

Institute for Fermentation, Osaka

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02491-21

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