Ultrasensitive and Specific Identification of Monkeypox Virus Congo Basin and West African Strains Using a CRISPR/Cas12b-Based Platform-Reference-Cited by-同舟云学术

Ultrasensitive and Specific Identification of Monkeypox Virus Congo Basin and West African Strains Using a CRISPR/Cas12b-Based Platform

Published:2023-04-13 Issue:2 Volume:11 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Chen Xu¹²^ORCID,Yuan Wei³,Yang Xinggui⁴,Shi Yuanfang¹,Zeng Xiaoyan¹²,Huang Junfei⁴,Wang Yi⁵,Li Shijun⁴

Affiliation:

1. The Second Clinical College, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, People’s Republic of China

2. Clinical Medical Laboratory of the Second Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, People’s Republic of China

3. Department of Quality Control, Guizhou Provincial Center for Clinical Laboratory, Guiyang, Guizhou, People’s Republic of China

4. Guizhou Provincial Centre for Disease Control and Prevention, Guiyang, Guizhou, People’s Republic of China

5. Experimental Research Center, Capital Institute of Pediatrics, Beijing, People’s Republic of China

Abstract

Monkeypox (MPX), a reemerging zoonotic disease caused by monkeypox virus (MPXV), causes a smallpox-like disease in humans. Early diagnosis is critical to prevent MPX epidemics. Here, CRISPR/Cas12b was integrated with LAMP amplification to devise a novel CRISPR-MPXV approach to achieve highly specific, ultrasensitive, rapid, and visual identification of MPXV Congo Basin and West African strains.

Funder

贵州省科技厅 | Science and Technology Program of Guizhou Province

The Program of Science and Technology of Guizhou Provincial Health Commission

The Scientific and Technological in Guiyang City

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.04035-22

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1. Reemergence of Human Monkeypox and Declining Population Immunity in the Context of Urbanization, Nigeria, 2017–2020