Human Herpesvirus 8 Serological Markers and Viral Load in Patients with AIDS-Associated Kaposi's Sarcoma in Central African Republic-Reference-Cited by-同舟云学术

Human Herpesvirus 8 Serological Markers and Viral Load in Patients with AIDS-Associated Kaposi's Sarcoma in Central African Republic

Published:2005-09 Issue:9 Volume:43 Page:4840-4843
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Duprez Renan¹,Kassa-Kelembho Eric²,Plancoulaine Sabine³,Brière Josette⁴,Fossi Mireille²,Kobangue Léon⁵,Minsart Pierre⁶,Huerre Michel⁷,Gessain Antoine¹

Affiliation:

1. Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Département Ecosystèmes et Epidémiologie des Maladies Infectieuses, Institut Pasteur, Paris, France

2. Institut Pasteur de Bangui, Bangui, Central African Republic

3. Laboratoire de Génétique Humaine des Maladies Infectieuses, INSERM U.550, Faculté de Médecine Necker, Paris, France

4. Laboratoire d'Anatomo-pathologie, Hopital Saint-Louis, Paris, France

5. Centre National Hospitalier Universitaire de Bangui, Bangui, Central African Republic

6. Hopital Communautaire de Bangui, Bangui, Central African Republic

7. Unité de Recherche et d'Expertise Histotechnologie et Pathologie, Institut Pasteur, Paris, France

Abstract

ABSTRACT Epidemic Kaposi's sarcoma (KS) is one of the most frequent types of cancer in several African countries; however, very few data are available on human herpesvirus 8 (HHV-8) markers in KS patients from Central Africa. In a series of 36 AIDS-KS cases from Central African Republic, we showed, using a real-time PCR quantitative assay, the high frequency (82%) of detectable HHV-8 DNA in peripheral blood mononuclear cells (PBMCs). We also found that the level of antibodies directed against lytic or latent HHV-8 antigens is not correlated to the amount of HHV-8 viral load in the PBMCs, and finally, we demonstrated a much higher viral load in tumoral skin lesions (6.07 log copies/μg DNA) than in unaffected skin (2.93 log copies/μg DNA) or in PBMCs (2.55 log copies/μg DNA).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.9.4840-4843.2005

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