Purification of staphylococcal alpha-toxin by adsorption chromatography on glass-Reference-Cited by-同舟云学术

Purification of staphylococcal alpha-toxin by adsorption chromatography on glass

Published:1976-03 Issue:3 Volume:13 Page:982-986
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Cassidy P,Harshman S

Abstract

Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2% of other forms.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.13.3.982-986.1976

Reference14 articles.

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4. CPG controlled-pore glass operation instructions. Electro-Nucleonics Inc. Fairfield N. J.

5. Disc electrophoresis-II, method and application to human serum proteins;Davis B. J.;Ann. N. Y. Acad. Sci.,1964

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