Specific Dot-Immunobinding Assay for Detection and Enumeration of Thiobacillus ferrooxidans-Reference-Cited by-同舟云学术

Specific Dot-Immunobinding Assay for Detection and Enumeration of Thiobacillus ferrooxidans

Published:1989-08 Issue:8 Volume:55 Page:2025-2029
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Arredondo Renato¹,Jerez Carlos A.¹

Affiliation:

1. Departamento de Bioqu�mica, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 7, Chile

Abstract

A specific and very sensitive dot-immunobinding assay for the detection and enumeration of the bioleaching microorganism Thiobacillus ferrooxidans was developed. Nitrocellulose spotted with samples was incubated with polyclonal antisera against whole T. ferrooxidans cells and then in 125 I-labeled protein A or 125 I-labeled goat antirabbit immunoglobulin G; incubation was followed by autoradiography. Since a minimum of 10 3 cells per dot could be detected, the method offers the possibility of simultaneous processing of numerous samples in a short time to monitor the levels of T. ferrooxidans in bioleaching operations.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.55.8.2025-2029.1989

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