Purification and Properties of Ornithine Racemase from Clostridium sticklandii-Reference-Cited by-同舟云学术

Purification and Properties of Ornithine Racemase from Clostridium sticklandii

Published:2000-04 Issue:7 Volume:182 Page:2052-2054
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Chen Hao-Ping¹,Lin Chin-Fen¹,Lee Ya-Jung²³,Tsay San-San⁴,Wu Shih-Hsiung³

Affiliation:

1. Department of Biochemistry, China Medical College, Taichung 404,1

2. Department of Food Science, National Chung-Hsiung University, Taichung 402,2

3. Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 115,3 Taiwan

4. Department of Botany, National Taiwan University, Taipei 106,4 and

Abstract

ABSTRACT Ornithine racemase has been purified to homogeneity from Clostridium sticklandii , as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This is the first racemase known to be highly specific to ornithine. This PLP-dependent enzyme has an M r of 92,000, with a K m for l -ornithine of 0.77 ± 0.05 mM and a k cat of 980 ± 20 s −1 .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.182.7.2052-2054.2000

Reference9 articles.

1. Andreesen J. R. Bahl H. Gottschalk G. Introduction to the physiology and biochemistry of the genus Clostridium Clostridia. Mintoz N. P. Clarke D. P. 1989 49 50 Plenum Press New York N.Y

2. Amino acid degradation by anaerobic bacteria;Barker H. A.;Ann. Rev. Biochem.,1981

3. Ornithine degradation in Clostridium sticklandii; pyridoxal phosphate and coenzyme A dependent thiolytic cleavage of 2-amino-4-ketopentanoate to alanine and acetyl coenzyme A;Jeng I. M.;Biochemistry,1974

4. A new amino acid racemase with threonine alpha-epimerase activity from Pseudomonas putida: purification and characterization

5. Reaction mechanism and structure of the active site of proline racemase;Rudnick G.;Biochemistry,1975

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