Sml1 Inhibits the DNA Repair Activity of Rev1 in Saccharomyces cerevisiae during Oxidative Stress

Abstract

Rev1 was critical for cell growth in S. cerevisiae , and the deletion of REV1 caused a severe growth defect in cells exposed to oxidative stress (2 mM H 2 O 2 ). Furthermore, we found that Sml1 physically interacted with Rev1 and inhibited Rev1 phosphorylation, thereby inhibiting Rev1 DNA antioxidant activity. These findings indicate that Sml1 could be a novel regulator for Rev1 in response to DNA damage by oxidative stress.