Affiliation:
1. Department of Food and Environmental Hygiene, College of Veterinary Medicine, Finland.
Abstract
A rapid agar diffusion method for the detection of DNase production of Campylobacter jejuni, C. coli, and C. pylori was developed. A strong pink zone indicating DNA hydrolysis was seen around the wells after 20 to 24 h of aerobic incubation at 37 degrees C. Pretreatment of cells with polymyxin B, which releases the cell-associated DNase, both shortened the time needed to read positive results to 8 h and increased the zone size.
Publisher
American Society for Microbiology
Reference10 articles.
1. Polymyxin B-induced release of low-molecular weight, heat-labile enterotoxin from Escherichia coli;Evans D. J.;Infect. Immun.,1974
2. 30 years of campylobacters: biochemical characteristics and a biotyping proposal for Campylobacterjejuni;Hébert G. A.;J. Clin. Microbiol.,1982
3. Methyl green. III. Reaction with deoxyribonucleic acid, stoichiometry, and behaviour of the reaction product;Kurnick N. B.;J. Gen. Physiol.,1950
4. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Apple;Lachica R. V. F.;Microbiol.,1971
5. Convenient assay for staphylococcal nuclease by the metachromatic well-agar-diffusion technique. Apple;Lachica R. V. F.;Microbiol.,1972
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