Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Infants by Use of Dried Blood Spots and an Ultrasensitive p24 Antigen Assay-Reference-Cited by-同舟云学术

Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Infants by Use of Dried Blood Spots and an Ultrasensitive p24 Antigen Assay

Published:2009-02 Issue:2 Volume:47 Page:459-462
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cachafeiro Ada¹,Sherman Gayle G.²,Sohn Annette H.³,Beck-Sague Consuelo⁴,Fiscus Susan A.¹

Affiliation:

1. Department of Microbiology & Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

2. Department of Molecular Medicine and Haematology, University of Witwatersrand, Johannesburg, South Africa

3. Division of Pediatric Infectious Diseases, University of California, San Francisco, San Francisco, California

4. Clínica de Familia MIR, La Romana, Dominican Republic

Abstract

ABSTRACT We tested 617 dried blood spots (DBS) from human immunodeficiency virus-exposed infants from five countries using an ultrasensitive p24 antigen assay (Up24). The sensitivity was 94.4% (67/71) and the specificity was 100% (431/431) for infants with DBS specimens ≤20 months old; DBS older than 30 months demonstrated only 72.2% sensitivity (39/54) ( P < 0.001) but displayed 100% specificity (61/61).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01181-08

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