Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene-Reference-Cited by-同舟云学术

Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene

Published:1995-05 Issue:5 Volume:33 Page:1375-1377
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Gunzburg S T¹,Tornieporth N G¹,Riley L W¹

Affiliation:

1. Department of Medicine, Cornell University Medical College, New York, New York 10021, USA.

Abstract

A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.33.5.1375-1377.1995

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