Identification of Campylobacter fetus by PCR-DNA probe method-Reference-Cited by-同舟云学术

Identification of Campylobacter fetus by PCR-DNA probe method

Published:1995-05 Issue:5 Volume:33 Page:1360-1362
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Blom K¹,Patton C M¹,Nicholson M A¹,Swaminathan B¹

Affiliation:

1. Foodborne and Diarrheal Diseases Branch, National Center for Infectious Diseases, Atlanta, Georgia 30333, USA.

Abstract

A PCR method for rapid identification of Campylobacter fetus subsp. fetus was evaluated. A fragment of the gene coding for 16S rRNA was amplified from crude cell lysates of 18 C. fetus strains and 30 strains representing other Campylobacter species and subspecies. The amplicons were probed by dot blot hybridization with a digoxigenin-labeled C. fetus-specific oligonucleotide probe. The probe reacted only with C. fetus subsp. fetus and C. fetus subsp. venerealis and may be useful for rapid identification in clinical laboratories.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.33.5.1360-1362.1995

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