Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene-Reference-Cited by-同舟云学术

Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene

Published:1995-05 Issue:5 Volume:33 Page:1344-1347
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Battles J K¹,Williamson J C¹,Pike K M¹,Gorelick P L¹,Ward J M¹,Gonda M A¹

Affiliation:

1. Laboratory of Cell and Molecular Structure, Program Resources, Inc./DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.

Abstract

Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridization assay. This assay was shown to be both sensitive and specific for H. hepaticus 16S rRNA gene sequences.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.33.5.1344-1347.1995

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