A Riboswitch Regulates Expression of the Coenzyme B 12 -Independent Methionine Synthase in Mycobacterium tuberculosis : Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551-Reference-Cited by-同舟云学术

A Riboswitch Regulates Expression of the Coenzyme B 12 -Independent Methionine Synthase in Mycobacterium tuberculosis : Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551

Published:2007-05 Issue:9 Volume:189 Page:3655-3659
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Warner Digby F.¹,Savvi Suzana¹,Mizrahi Valerie¹,Dawes Stephanie S.¹

Affiliation:

1. MRC/NHLS/WITS Molecular Mycobacteriology Research Unit and DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, School of Pathology, University of the Witwatersrand and the National Health Laboratory Service, Johannesburg, South Africa

Abstract

ABSTRACT We observed vitamin B 12 -mediated growth inhibition of Mycobacterium tuberculosis strain CDC1551. The B 12 sensitivity was mapped to a polymorphism in metH , encoding a coenzyme B 12 -dependent methionine synthase. Vitamin B 12 -resistant suppressor mutants of CDC1551 containing mutations in a B 12 riboswitch upstream of the metE gene, which encodes a B 12 -independent methionine synthase, were isolated. Expression analysis confirmed that the B 12 riboswitch is a transcriptional regulator of metE in M. tuberculosis .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00040-07

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