Generation and Evaluation of a Glaesserella (Haemophilus) parasuis Capsular Mutant-Reference-Cited by-同舟云学术

Generation and Evaluation of a Glaesserella (Haemophilus) parasuis Capsular Mutant

Published:2020-04-20 Issue:5 Volume:88 Page:
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Eberle Kirsten C.¹,Hau Samantha J.¹,Luan Shi-Lu²,Weinert Lucy A.²,Stasko Judith A.¹,Wang Jinhong²,Peters Sarah E.²,Langford Paul R.³^ORCID,Rycroft Andrew N.⁴,Wren Brendan W.⁵,Maskell Duncan J.²,Tucker Alexander W.²,Brockmeier Susan L.¹

Affiliation:

1. National Animal Disease Center, Agricultural Research Service, USDA, Ames, Iowa, USA

2. Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

3. Section of Paediatric Infectious Disease, Department of Infectious Disease, Imperial College London, St. Mary’s Campus, London, United Kingdom

4. The Royal Veterinary College, Hawkshead Campus, Hatfield, United Kingdom

5. Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom

Abstract

Glaesserella ( Haemophilus ) parasuis is a commensal bacterium of the upper respiratory tract in pigs and also the causative agent of Glässer’s disease, which causes significant morbidity and mortality in pigs worldwide. Isolates are characterized into 15 serovars by their capsular polysaccharide, which has shown a correlation with isolate pathogenicity. To investigate the role the capsule plays in G. parasuis virulence and host interaction, a capsule mutant of the serovar 5 strain HS069 was generated (HS069Δcap) through allelic exchange following natural transformation.

Funder

Wellcome Trust

UK Research and Innovation | Biotechnology and Biological Sciences Research Council

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.00879-19

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