Interaction between SWP9 and Polar Tube Proteins of the Microsporidian Nosema bombycis and Function of SWP9 as a Scaffolding Protein Contribute to Polar Tube Tethering to the Spore Wall

Author:

Yang Donglin123,Pan Lixia4,Peng Pai15,Dang Xiaoqun6,Li Chunfeng15,Li Tian15,Long Mengxian15,Chen Jie15,Wu Yujiao15,Du Huihui15,Luo Bo15,Song Yue15,Tian Rui15,Luo Jie135,Zhou Zeyang165,Pan Guoqing15

Affiliation:

1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, People's Republic of China

2. International Academy of Targeted Therapeutics and Innovation, Chongqing Key Laboratory of Kinase Modulators as Innovative Medicine, Chongqing Engineering Laboratory of Targeted and Innovative Therapeutics, Chongqing University of Arts and Sciences, Chongqing, People's Republic of China

3. College of Forestry and Life Sciences, Chongqing University of Arts and Sciences, Chongqing, People's Republic of China

4. Foreign Language Department, Chongqing Water Resources and Electric Engineering College, Chongqing, People's Republic of China

5. Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, People's Republic of China

6. College of Life Sciences, Chongqing Normal University, Chongqing, People's Republic of China

Abstract

ABSTRACT All microsporidia possess a unique, highly specialized invasion mechanism that involves the polar tube and spore wall. The interaction between spore wall proteins (SWPs) and polar tube proteins (PTPs) in the formation, arrangement, orderly orientation, and function of the polar tube and spore wall remains to be determined. This study was undertaken to examine the protein interactions of Nosema bombycis SWP7 (NbSWP7), NbSWP9, and PTPs. Coimmunoprecipitation, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and yeast two-hybrid data demonstrated that NbSWP9, but not NbSWP7, interacts with NbPTP1 and NbPTP2. Furthermore, immunoelectron microscopy (IEM) showed that NbSWP9 was localized mainly in the developing polar tube of sporoblasts, while NbSWP7 was found randomly in the cytoplasm. However, both NbSWP9 and NbSWP7 were located in the polar tube and spore wall of N. bombycis mature spores. The reason why NbSWP7 was localized to the polar tube may be due to the interaction between NbSWP9 and NbSWP7. Interestingly, the majority of NbSWP9, but not NbSWP7, accumulated in the beginning part of the extruded polar tube and the ruptured spore wall called the anchoring disk (AD) when the mature spores germinated under weak-alkaline environmental stimulation. Additionally, anti-NbSWP9 antibody reduced spore germination in a dose-dependent manner. In conclusion, our study further confirmed that NbSWP9 is a scaffolding protein that not only anchors and holds the polar tube but also tethers the polar tube to the spore wall.

Funder

Project of National Basic Research Program of China

National Natural Science Foundation of China

Chongqing Science and Technology Commission

Chongqing Municipal Education Commission

Chongqing University of Arts and Sciences

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference45 articles.

1. Becnel JJ Andreadis TG . 1999. Microsporidia in insects, p 447–501. InWittnerMWeissLM (ed), The microsporidia and microsporidiosis. ASM Press, Washington, DC.

2. Microsporidiosis: An emerging and opportunistic infection in humans and animals

3. Uber die neue Krankheit der Seidenraupe und verwandte Organismen;Nägeli K;Bot Z,1857

4. Microsporidia: Biology and Evolution of Highly Reduced Intracellular Parasites

5. Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi

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