Transcription of the Influenza Ribonucleic Acid Genome by a Virion Polymerase II. Nature of the In Vitro Polymerase Product-Reference-Cited by-同舟云学术

Transcription of the Influenza Ribonucleic Acid Genome by a Virion Polymerase II. Nature of the In Vitro Polymerase Product

Published:1971-07 Issue:1 Volume:8 Page:74-80
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Bishop David H. L.¹²,Obijeski John F.¹²,Simpson Robert W.¹²

Affiliation:

1. Institute of Cancer Research, Columbia University, New York, New York 10032

2. Institute of Microbiology, Rutgers University, New Brunswick, New Jersey 08903

Abstract

The properties of the ribonucleic acid (RNA) synthesized by the influenza (WSN) virion polymerase have been investigated. Most of the product RNA is synthesized in association with virion RNA species from which it can be released by heat treatment as single-stranded, ribonuclease-sensitive polynucleotides (average molecular weight, 2-hr sample, about 10 5 daltons). At least 95% of the product is complementary to the viral RNA species. On the basis of the molar ratio of the RNA species isolated from a 3 H-uridine-labeled virus preparation, it was calculated that the WSN genome consists of seven pieces of RNA with a sum molecular weight of about 5 × 10 6 daltons.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.8.1.74-80.1971

Reference18 articles.

1. Viral RNA-dependent DNA polymerase;Baltimore D.;Nature (London),1970

2. Ribonucleic acid synthesis of vesicular stomatitis virus. 1. An RNA polymerase in the virion;Baltimore D.;Proc. Nat. Acad. Sci. U.S.A.,1970

3. Complete transcription by the transcriptase of vesicular stomatitis virus;Bishop D. H. L.;J. Virol.,1971

4. Electrophoretic separation of viral nucleic acids on polyacrylarmide gels;Bishop D. H. L.;J. Mol. Biol.,1967

5. Transcription of the influenza ribonucleic acid genome by a virion polymerase. 1. Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase;Bishop D. H. L.;J. Virol.,1971

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