SARS-CoV-2 Testing in the Community: Testing Positive Samples with the TaqMan SARS-CoV-2 Mutation Panel To Find Variants in Real Time-Reference-Cited by-同舟云学术

SARS-CoV-2 Testing in the Community: Testing Positive Samples with the TaqMan SARS-CoV-2 Mutation Panel To Find Variants in Real Time

Published:2022-04-20 Issue:4 Volume:60 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ashford Fiona¹,Best Angus¹,Dunn Steven J.²,Ahmed Zahra¹,Siddiqui Henna¹,Melville Jordan¹,Wilkinson Samuel²,Mirza Jeremy²,Cumley Nicola²,Stockton Joanne²,Ferguson Jack³,Wheatley Lucy³,Ratcliffe Elizabeth⁴,Casey Anna⁴,Plant Tim¹,Quick Joshua²,Richter Alex¹,Loman Nicholas²,McNally Alan²^ORCID,

Affiliation:

1. Clinical Immunology Service, Institute of Immunology and Immunotherapy, College of Medical and Dental Science, University of Birmingham, Birmingham, United Kingdom

2. Institute of Microbiology and Infection, College of Medical and Dental Science, University of Birmingham, Birmingham, United Kingdom

3. Institute of Cancer and Genomic Science, College of Medical and Dental Science, University of Birmingham, Birmingham, United Kingdom

4. Department of Clinical Microbiology, University Hospitals Birmingham NHS Foundation Trust, Queen Elizabeth Hospital, Birmingham, United Kingdom

Abstract

Genome sequencing is a powerful tool for identifying SARS-CoV-2 variant lineages; however, there can be limitations due to sequence dropout when used to identify specific key mutations. Recently, ThermoFisher Scientific has developed genotyping assays to help bridge the gap between testing capacity and sequencing capability to generate real-time genotyping results based on specific variants.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.02408-21

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