Direct Sequencing and RipSeq Interpretation as a Tool for Identification of Polymicrobial Infections-Reference-Cited by-同舟云学术

Direct Sequencing and RipSeq Interpretation as a Tool for Identification of Polymicrobial Infections

Published:2013-04 Issue:4 Volume:51 Page:1281-1284
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Wolff Tine Y.¹,Eickhardt Steffen²³,Björnsdottir Maria K.²,Moser Claus²,Bjarnsholt Thomas²³,Høiby Niels²,Thomsen Trine R.¹⁴

Affiliation:

1. The Danish Technological Institute, Life Science Division, Aarhus, Denmark

2. Department of Clinical Microbiology, Rigshospitalet, University Hospital of Copenhagen, Copenhagen, Denmark

3. Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark

4. Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Aalborg, Denmark

Abstract

ABSTRACT In this study, RipSeq Mixed, a software resolving uninterpretable mixed DNA sequencing chromatograms, revealed the bacterial content of 15 polymicrobial samples. Direct sequencing combined with RipSeq Mixed constitutes a valuable supplement to cultivation, particularly when cultivation is negative and direct sequencing is inconclusive despite continued clinical indications of infection.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00190-13

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