Cross-reactivity of polyclonal serum antibodies generated against Cryptosporidium parvum oocysts

Author:

Ortega-Mora L M1,Troncoso J M1,Rojo-Vázquez F A1,Gómez-Bautista M1

Affiliation:

1. Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.

Abstract

Polyclonal antibodies raised against Cryptosporidium parvum oocysts were found to cross-react with Eimeria spp. oocyst antigens in an indirect immunofluorescence assay, and sera from Eimeria spp.-infected lambs reacted with some antigens from sonicated C. parvum oocysts (between 29 to 30 and 66 to 69 kDa) by Western blot (immunoblot). No cross-reaction was observed with cystozoites of Toxoplasma and Sarcocystis spp. These results show the existence of epitopes common to C. parvum and various Eimeria spp.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference30 articles.

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2. Effects of immune colostrum and orally administered antisporozoite monoclonal antibodies on the outcome of Cryptosponidiumparvum infections in neonatal mice;Arrowood M. J.;Infect. Immun.,1989

3. Cryptosporidium parvum merozoites share neutralization-sensitive epitopes with sporozoites;Bjorneby J. M.;J. Immunol.,1990

4. Demonstration of serum antibodies to Cryptosponidium sp. in normal and immunodeficient humans with confirmed infections;Campbell P. N.;J. Clin. Microbiol.,1983

5. Catty D. and C. Raykundalia. 1989. ELISA and related enzyme immunoassays p. 97-152. In D. Catty (ed.) Antibodies a practical approach vol. II. IRL Press Oxford.

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