Analysis of ochratoxin B alone and in the presence of ochratoxin A, using carboxypeptidase A

Author:

Hult K,H�kby E,Gatenbeck S

Abstract

A method is described for ochratoxin B analysis, which is adapted to the earlier described method of ochratoxin A analysis, using carboxypeptidase A (K. Hult and S. Gatenbeck, J. Assoc. Off. Anal. Chem. 59:128-129, 1976). The fluorescence spectra of ochratoxins A and B coincide too much to allow direct discrimination of the two compounds. A method using the differences in kinetic parameters of the enzymatic hydrolysis of the two compounds is suggested for the analysis of mixtures of the ochratoxins.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference3 articles.

1. Structural requirements for ochratoxin intoxication;Chu F. S.;Life Sci.,1972

2. Comparative rates of hydrolysis of ochratoxin A and B in vitro;Doster R. C.;Food Cosmet. Toxicol.,1972

3. A spectrophotometric procedure, using carboxypeptidase A, for the quantitative measurement of ochratoxin A;Hult K.;J. Assoc. Off. Anal. Chem.,1976

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