Affiliation:
1. Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301.
Abstract
Treatment of toxoplasmic encephalitis in C57BL/6 mice with monoclonal antibody (MAb) against interleukin-6 (IL-6) resulted in a remarkable decrease in the number of foci of acute inflammation in their brains caused by proliferation of tachyzoites. In brains of mice treated with isotype control MAb and those treated with anti-IL-6 MAb, tachyzoites were observed only in foci of acute inflammation. Immunoperoxidase staining revealed a greatly diminished frequency of tachyzoites in brains of mice treated with anti-IL-6 MAb. Of interest, treatment with MAb against IL-6 was also associated with reduced numbers of Toxoplasma gondii cysts in the brains and with higher serum levels of gamma interferon than in control mice. Paradoxically, the mice treated with anti-IL-6 MAb had higher serum levels of IL-6 as measured by an enzyme-linked immunosorbent assay than controls. These results revealed the importance of IL-6 in the immunopathogenesis of murine toxoplasmic encephalitis.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference17 articles.
1. Beaman M. H. J. S. Abrams and J. S. Remington. 1993. Unpublished data.
2. Two types of mouse T cell clone: further differences in Iymphokine synthesis between TH1 and TH2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies;Cherwinski H. M.;J. Exp. Med.,1987
3. Toxoplasma gondii infection of the central nervous system: use of the peroxidase-antiperoxidase method to demonstrate toxoplasma in formalin-fixed paraffin embedded tissue sections;Conley F. K.;Hum. Pathol.,1981
4. Simultaneous depletion of CD4+ and CD8+ T Iymphocytes is required to reactivate chronic infection with Toxoplasma gondii;Gazzinelli R.;J. Immunol.,1992
5. Acute cerebral toxoplasmosis is induced by in vivo neutralization of TNF-a and correlates with the down-regulated expression of inducible nitric oxide synthase and other markers of macrophage activation;Gazzinelli R. T.;J. Immunol.,1993
Cited by
55 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献