Affiliation:
1. Process Development Division, Department of the Army, Fort Detrick, Frederick, Maryland 27101
Abstract
Procedures used to produce an established line of mammalian cells (L cell) in a New Brunswick fermentor were adapted to propagate human lymphoid cells in suspended culture. Control of
p
H within defined limits was more effective for regulation of cell metabolism than control of oxidation-reduction potential. An unusually high rate of agitation was required.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine