Affiliation:
1. Soil Microbiology Laboratory, Istituto Sperimentale per le Colture Industriali, Via di Corticella n. 133, 40129 Bologna, Italy
Abstract
The fungus
Polymyxa betae
Keskin belongs to the family
Plasmodiophoraceae
and lives in the soil as an obligatory parasite of the roots of the
Chenopodiaceae
. When contaminated by beet necrotic yellow vein virus, this viruliferous fungus causes a serious disease of sugar beet known as rhizomania, whereas the infection by the fungus alone (aviruliferous fungus) causes only slight damage to the plant with little economic consequence. The manifestation of rhizomania in sugar beet is directly related to the concentration of infecting units of viruliferous
P. betae
present in the soil. (One infecting unit is a group of one or more sporosori that liberate zoospores capable of visibly infecting a plant.) By using current methods of analysis, it is possible to estimate the total quantity of
P. betae
present in the soil, but one cannot distinguish quantitatively the infecting units of aviruliferous from viruliferous
P. betae
. A new method has been developed based on the technique of the most probable number and enzyme-linked immunosorbent assay to estimate the concentration of infecting units of viruliferous
P. betae
in soil. The method is suitable for the routine analysis of numerous soil samples and allows one to estimate the concentration of viable forms of the fungus
P. betae
, whether or not contaminated by beet necrotic yellow vein virus, present in a soil affected by rhizomania or presumed healthy. The analyses performed with this method are economical and use a reagent kit and equipment in wide use.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
14 articles.
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