Development of a Singleplex PCR Assay for Rapid Identification and Differentiation of Cryptococcus neoformans var. grubii , Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and Hybrids-Reference-Cited by-同舟云学术

Development of a Singleplex PCR Assay for Rapid Identification and Differentiation of Cryptococcus neoformans var. grubii , Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and Hybrids

Published:2013-06 Issue:6 Volume:51 Page:1920-1923
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Feng Xiaobo¹,Fu Xiaohua²,Ling Bo¹,Wang Lei²,Liao Wanqing³,Yao Zhirong¹

Affiliation:

1. Medical Mycology Laboratory, Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine

2. School of Environmental Science and Engineering, Tongji University

3. Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Institute of Medical Mycology, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai, China

Abstract

ABSTRACT A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii , Cryptococcus neoformans var. neoformans , and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybrids were also characterized on the basis of distinct combined profiles of amplicons. This PCR assay is a rapid, simple, and reliable approach suitable for laboratory diagnoses and large-scale epidemiologic studies.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00064-13

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