Analysis of Certain Variables in the Agar Dilution Susceptibility Test

Author:

Snyder Robert J.1,Kohner Peggy C.1,Ilstrup Duane M.1,Washington John A.1

Affiliation:

1. Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901

Abstract

This study examined (i) the activity of gentamicin added to agar in ratios of 1:100 and 1:1,000 to produce a final concentration of 1 μg/ml, (ii) the uniformity of distribution of gentamicin in agar in relation to the amount of mixing, and (iii) the effect of agar depth on the activity of gentamicin against Pseudomonas aeruginosa . Although the ratio of antibiotic solution to agar had no significant effects on activity of the antibiotic, the amount of mixing did. Agar depth had no significant effect on the activity of gentamicin.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference6 articles.

1. Cooper K. E. 1963. The theory of antibiotic inhibition zones p. 4-38. In F. Kavanagh (ed.) Analytical microbiology. Academic Press Inc. New York.

2. Antibiotic sensitivity testing- report of an international collaborative study. Acta;Ericson H. M.;Pathol. Microbiol. Scand. Sect. B Suppl.,1971

3. An inocula replicating apparatus for routine testing of bacterial susceptibility to antibiotics;Steers E.;Antibiot. Chemother. (Basel),1969

4. Washington J. A. II (ed.). 1974. Laboratory procedures in clinical microbiology p. 286-297. Little Brown and Co. Boston.

5. Washington J. A. II and A. L. Barry. 1974. Dilution test procedures p. 410-417. In E. H. Lennette E. H. Spaulding and J. P. Truant (ed.) Manual of clinical microbiology 2nd ed. American Society for Microbiology Washington D.C.

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