Affiliation:
1. Division of Experimental Chemotherapy, Sloan-Kettering Institute for Cancer Research, and Sloan-Kettering Division, Graduate School of Medical Sciences, Cornell University Medical College, New York, New York
Abstract
Stonehill
, E. H. (Sloan-Kettering Institute for Cancer Research, New York, N.Y.
and Dorris J. Hutchison
. Chromosomal mapping by means of mutational induction in synchronous populations of
Streptococcus faecalis
. J. Bacteriol.
92:
136–143. 1966.—A new method for mapping genetic loci on the bacterial chromosome, based on the linear progression of the deoxyribonucleic acid-replicating enzyme (replicase) in synchronous cultures of
Streptococcus faecalis
, is described. Ultraviolet irradiation was used to induce mutations to drug and to bacteriophage resistance at various intervals during the progress of the replicase along the chromosome. The time of duplication of the gene in synchronous populations was determined from reductions in the rates of irradiation-induced mutation. After the replication of various genes, the rates fell to half their former values. The resulting data made possible the construction of a 1-hr time-map indicating the sequence of replication of five genes in
S. faecalis
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
23 articles.
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