Rates of Microbial Transformation of Polycyclic Aromatic Hydrocarbons in Water and Sediments in the Vicinity of a Coal-Coking Wastewater Discharge

Abstract

To facilitate predictions of the transport and fate of contaminants at future coal conversion facilities, rates of microbial transformation of polycyclic aromatic hydrocarbons were measured in stream water and sediment samples collected in the vicinity of a coal-coking treated wastewater discharge from November 1977 through August 1979. Six radiolabeled polycyclic aromatic hydrocarbons were incubated with sediment and water samples; 14 CO 2 , cell-bound 14 C, and polar transformation products were isolated and quantified. Whereas 14 CO 2 and bound 14 C were major transformation products in sediment assays, soluble polar 14 C dominated transformation in water samples. Mean rate constants (measured at 20°C) in sediments collected downstream from the effluent outfall were 7.8 × 10 −2 h −1 (naphthalene), 1.6 × 10 −2 h −1 (anthracene), and 3.3 × 10 −3 h −1 [benz(a)anthracene], which corresponded to turnover times of 13, 62, and 300 h, respectively. No unequivocal evidence for transformation of benzo(a)pyrene or dibenz(a,h)anthracene was obtained. Only naphthalene and anthracene transformations were observed in water samples; rate constants were consistently 5- and 20-fold lower, respectively, than in the corresponding sediment samples. The measured rate constants for anthracene transformation in July 1978 sediment samples were not related to total heterotroph numbers. In late July 1978, the effluent was diverted from the primary study area; however, no differences were observed either in transformation rate constants or in the downstream/upstream sediment rate constant ratio. These results are consistent with the hypothesis that continuous inputs of polycyclic aromatic hydrocarbons result in an increased ability within a microbial community to utilize certain polycyclic aromatic hydrocarbons. However, because transformation rates remained elevated for more than 1 year after removal of the polycyclic aromatic hydrocarbon source, microbial communities may shift only slowly in response to changes in polycyclic aromatic hydrocarbon concentrations.