Fusion of Large Polypeptides to Human Adenovirus Type 5 Capsid Protein IX Can Compromise Virion Stability and DNA Packaging Capacity-Reference-Cited by-同舟云学术

Fusion of Large Polypeptides to Human Adenovirus Type 5 Capsid Protein IX Can Compromise Virion Stability and DNA Packaging Capacity

Published:2020-08-17 Issue:17 Volume:94 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Poulin Kathy L.¹,McFall Emily R.¹²³,Chan Grace¹,Provost Natacha B.¹,Christou Carin¹,Smith Adam C.¹,Parks Robin J.¹²³⁴^ORCID

Affiliation:

1. Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

2. Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada

3. Centre for Neuromuscular Disease, University of Ottawa, Ottawa, Ontario, Canada

4. Department of Medicine, University of Ottawa, Ottawa, Ontario, Canada

Abstract

Human adenovirus capsid protein IX (pIX) is involved in stabilizing the virion but has also been developed as a platform for presentation of various polypeptides on the surface of the virion. Whether such modifications affect the ability of pIX to stabilize the virion is unknown. We show that addition of large polypeptides to pIX can reduce both the DNA packaging capacity and the heat stability of the virion, which provides important guidance for the design of pIX-modified vectors.

Funder

Ontario Provincial Government

Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada

Gouvernement du Canada | Canadian Institutes of Health Research

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01112-20

Reference56 articles.

1. Characterization of Adenovirus Protein IX