Concentration of viruses from water by using cellulose filters modified by in situ precipitation of ferric and aluminum hydroxides

Author:

Farrah S R,Preston D R

Abstract

Untreated cellulose filters adsorbed only small amounts of poliovirus 1, echovirus 5, coxsackievirus B5, or bacteriophage MS2 that were added to tap water or to solutions of imidazole-glycine buffer at pH 5 to 7. Modification of filters by in situ flocculation of ferric and aluminum hydroxides greatly increased the ability of the filters to adsorb viruses. Viruses adsorbed to the modified filters could be recovered by treating the filters with 3% beef extract (pH 9.5). Greater than 60% of the enteroviruses and greater than 55% of the MS2 added to tap water or buffer could be recovered in the beef extract eluate.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference16 articles.

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2. Farrah S. R. and G. Bitton. 1982. Methods (other than microporous filters) for concentration of viruses from water. In C. P. Gerba and S. M. Goyal (ed.) Methods in environmental virology. Marcel Dekker Inc. New York.

3. Concentration of viruses from large volumes of tap water using pleated membrane filters;Farrah S. R.;Appl. Environ. Microbiol.,1976

4. Applied and theoretical aspects of virus adsorption to surfaces;Gerba C. P.;Adv. Appl. Microbiol.,1984

5. Goyal S. M. and C. P. Gerba. 1981. Membrane filters in virology. In B. J. Dutka (ed.) Membrane filtration. Marcel Dekker Inc. New York.

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