Transfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression

Author:

Bourgouin C1,Del�cluse A1,de la Torre F1,Szulmajster J1

Affiliation:

1. Unit� de Biochimie Microbienne, Institut Pasteur, Paris, France.

Abstract

The genes encoding the toxic determinants of Bacillus sphaericus have been expressed in a nontoxic and a toxic strain of Bacillus thuringiensis subsp. israelensis. In both cases, the B. sphaericus toxin proteins were produced at a high level during sporulation of B. thuringiensis and accumulated as crystalline structures. B. thuringiensis transformants expressing B. sphaericus and B. thuringiensis subsp. israelensis toxins did not show a significant enhancement of toxicity against Aedes aegypti, Anopheles stephensi, and Culex pipiens larvae.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference21 articles.

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5. Bourgouin C. J.-F. Charles A. R. Kalfon and H. de Barjac. 1984. Bacillus sphaericus 2297: purification and biogenesis of parasporal inclusions toxic for mosquito larvae p. 389-390. In J. Alouf et al. (ed.) Bacterial protein toxins. Academic Press Inc. (London) Ltd. London.

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