Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae-Reference-Cited by-同舟云学术

Characterization of the Phase-Variable Autotransporter Lav Reveals a Role in Host Cell Adherence and Biofilm Formation in Nontypeable Haemophilus influenzae

Published:2022-04-21 Issue:4 Volume:90 Page:
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Phillips Zachary N.¹,Garai Preeti²,Tram Greg¹,Martin Gael¹,Van Den Bergh Annelies¹,Husna Asma-Ul¹,Staples Megan³,Grimwood Keith⁴⁵⁶,Jennison Amy V.³^ORCID,Guillon Patrice¹,von Itzstein Mark¹^ORCID,Jennings Michael P.¹^ORCID,Brockman Kenneth L.²,Atack John M.¹⁷^ORCID

Affiliation:

1. Institute for Glycomics, Griffith University, Gold Coast, Queensland, Australia

2. Department of Microbiology & Immunology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA

3. Queensland Department of Health, Public Health Microbiology, Forensic and Scientific Services, Brisbane, Queensland, Australia

4. School of Medicine and Dentistry and Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia

5. Department of Infectious Diseases, Gold Coast Health, Gold Coast, Queensland, Australia

6. Department of Paediatrics, Gold Coast Health, Gold Coast, Queensland, Australia

7. School of Environment and Science, Griffith University, Gold Coast, Queensland, Australia

Abstract

Lav is an autotransporter protein found in pathogenic Haemophilus and Neisseria species. Lav in nontypeable Haemophilus influenzae (NTHi) is phase-variable: the gene reversibly switches ON-OFF via changes in length of a locus-located GCAA (n) simple DNA sequence repeat tract. The expression status of lav was examined in carriage and invasive collections of NTHi, where it was predominantly not expressed (OFF). Phenotypic study showed lav expression (ON) results in increased adherence to human lung cells and denser biofilm formation.

Funder

Australian Research Council

Department of Health | National Health and Medical Research Council

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.00565-21

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