Affiliation:
1. Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland, USA
Abstract
ABSTRACT
Many microbes exploit host cellular lipid droplets during the host-microbe interaction, but this phenomenon has not been extensively studied for fungal pathogens. In this study, we analyzed the role of lipid droplets during the interaction of
Cryptococcus neoformans
with macrophages in the presence and the absence of exogenous lipids, in particular, oleate. The addition of oleic acid increased the frequency of lipid droplets in both
C. neoformans
and macrophages
. C. neoformans
responded to oleic acid supplementation by faster growth inside and outside macrophages. Fungal cells were able to harvest lipids from macrophage lipid droplets. Supplementation of
C. neoformans
and macrophages with oleic acid significantly increased the rate of nonlytic exocytosis while having no effect on lytic exocytosis. The process for lipid modulation of nonlytic exocytosis was associated with actin changes in macrophages. In summary,
C. neoformans
harvests lipids from macrophages, and the
C. neoformans
-macrophage interaction is modulated by exogenous lipids, providing a new tool for studying nonlytic exocytosis.
Funder
HHS | NIH | National Heart, Lung, and Blood Institute
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
18 articles.
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