Estimation of the State of the Bacterial Cell Wall by Fluorescent In Situ Hybridization-Reference-Cited by-同舟云学术

Estimation of the State of the Bacterial Cell Wall by Fluorescent In Situ Hybridization

Published:1998-08 Issue:8 Volume:64 Page:3059-3062
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bidnenko Elena¹,Mercier Carine²,Tremblay Josselyne²,Tailliez Patrick²,Kulakauskas Saulius²

Affiliation:

1. Génétique Microbienne1 and

2. Unité de Recherches Laitières et de Génétique Appliquée,2 Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France

Abstract

ABSTRACT Fluorescent in situ hybridization (FISH) is now a widely used method for identification of bacteria at the single-cell level. With gram-positive bacteria, the thick peptidoglycan layer of a cell wall presents a barrier for entry of horseradish peroxidase (HRP)-labeled probes. Therefore, such probes do not give any signal in FISH unless cells are first treated with enzymes which hydrolyze the peptidoglycan. We explored this feature of FISH to detect cells which have undergone permeabilization due to expression of autolytic enzymes. Our results indicate that FISH performed with HRP-labeled probes provides a sensitive method to estimate the states of cell walls of individual gram-positive bacteria.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.64.8.3059-3062.1998

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