Solubilization of the Cytoplasmic Membrane of Escherichia coli by the Ionic Detergent Sodium-Lauryl Sarcosinate-Reference-Cited by-同舟云学术

Solubilization of the Cytoplasmic Membrane of Escherichia coli by the Ionic Detergent Sodium-Lauryl Sarcosinate

Published:1973-09 Issue:3 Volume:115 Page:717-722
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Filip Camille¹,Fletcher Gail¹,Wulff Judith L.¹,Earhart C. F.¹

Affiliation:

1. Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712

Abstract

The sensitivity of the outer and cytoplasmic membranes of Escherichia coli to detergent was examined by isopycnic sucrose density gradient centrifugation. Sodium lauryl sarcosinate (Sarkosyl) was found to disrupt the cytoplasmic membrane selectively under conditions in which Triton X-100 and dodecyl sodium sulfate solubilized all membrane protein. These results were verified by gel electrophoresis; membrane proteins solubilized by Sarkosyl were identical to those of the cytoplasmic membrane. The presence of Mg 2+ during treatment with Sarkosyl was found to afford partial protection of the cytoplasmic membrane from dissolution.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.115.3.717-722.1973

Reference18 articles.

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2. Some features of the bacterial membrane studied with the aid of new fractionation technique;Daniels M. J.;Biochem. J.,1971

3. Attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer, lipopolysaccharide membrane and the cytoplasmic membrane;De Piamphilis M. L.;J. Bacteriol.,1971

4. The association of host and phage DNA with the membrane of Escherichia coli;Earhart C. F.;Virology,1970

5. The topography of the bacterial cell wall. Annu;Glauert A. M.;Rev. Microbiol.,1969

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