Affiliation:
1. Idexx Corporation, Portland, Maine 04101.
Abstract
DNA probes that hybridize to a mycobacterial insertion sequence, IS900, present in multiple copies in the genome of Mycobacterium paratuberculosis were found to be highly specific for M. paratuberculosis. DNA sequences derived from IS900 were used to prepare DNA primers for detection and identification of M. paratuberculosis by the polymerase chain reaction. Highly specific direct detection of M. paratuberculosis DNA in feces from cattle with Johne's disease was obtained. The polymerase chain reaction test had a sensitivity equal to or greater than that obtained by standard culture techniques and was much more rapid, taking only hours compared with 6 to 12 weeks for culture.
Publisher
American Society for Microbiology
Reference21 articles.
1. Isolation, identification, and structural analysis of the mycobactins of Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, and Mycobacterium paratuberculosis;Barclay R.;J. Bacteriol.,1985
2. Immunological reactions caused by infection with M. paratuberculosis. A review;Bendixen P. H.;Nord. Veterinaermed.,1978
3. Nucleotide sequence of ISJJO, an insertion sequence from Streptomyces coelicolor A3(2);Bruton C. J.;Nucleic Acids Res.,1987
4. Ruminant paratuberculosis (Johne's disease). The current status and future prospects;Chiodini R. J.;Cornell Vet.,1984
5. Possible role of mycobacteria in inflammatory bowel disease. 1. An unclassified Mycobacterium species isolated from patients with Crohn's disease;Chiodini R. J.;Dig. Dis. Sci.,1984
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