Identification and elimination of DNA sequences in Taq DNA polymerase-Reference-Cited by-同舟云学术

Identification and elimination of DNA sequences in Taq DNA polymerase

Published:1994-08 Issue:8 Volume:32 Page:2007-2008
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hughes M S¹,Beck L A¹,Skuce R A¹

Affiliation:

1. Department of Agriculture for Northern Ireland, Stromont, Belfast.

Abstract

This study confirms that different preparations of Taq DNA polymerase are contaminated with eubacterial DNA. The contaminants appeared to represent more than one strain or species but were not identified as Thermus aquaticus or Escherichia coli. Differences in microcentrifuge tube composition appeared to affect elimination of the contaminants.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.32.8.2007-2008.1994

Reference5 articles.

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4. Taq polymerase contains bacterial DNA of unknown origin;Rand K. H.;Mol. Cell. Probes,1990

5. Detection of DNA contamination in Taq polymerase;Schmidt T. M.;BioTechniques,1991

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